PTH / parathyroid hormone (aa 32-65) Protein (GST Tag)

18.04.2012

Produkt-Information von PTH / parathyroid hormone Protein

Synonym	PTH1
Protein Construction	A DNA sequence encoding the human PTH (P05062) (Ser 32-Phe 65) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PTH / parathyroid hormone Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: PTH / parathyroid hormone protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PTH/GST chimera consists of 265 amino acids and has a predicted molecular mass of 31 kDa. It migrates as an approxiamtely 30 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PTH / parathyroid hormone Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTH / parathyroid hormone Protein

Parathyroid hormone (PTH), parathormone or parathyrin, is secreted by the chief cells of the parathyroid glands as a polypeptide. PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. PTH may stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Parathyroid hormone (PTH) has been proved to play a pivotal role in maintaining myocardial contractility as well as effective natriuresis, and possible pathogenic mechanisms contributing to heart failure secondary to hypocalcemia and hypoparathyroidism. With the increased population of preosteoblastic lineages and the osteoblastic activation, Parathyroid hormone (PTH) drives anabolism in bone. Experiments have recently reported that the absence of osteoclasts in c-fos(- / -) mice might hinder PTH-driven bone anabolism, and that osteoclastic presence may be necessary for full osteoblastic differentiation and enhanced bone formation seen after intermittent PTH administration. Alternatively, it was suggested that PTH administration inhibits sclerostin synthesis by osteocytes, thereby allowing for active bone formation. Taken together, Parathyroid hormone (PTH) affects bone cells in a dual pathway-mediating osteoblastic (preosteoblastic) activities or osteocytic synthesis of sclerostin. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone.

References

Bedi B. et al., 2012, Proc Natl Acad Sci. 109 (12): 725-33.
Hasegawa T. et al., 2012, Clin Calcium. 22 (3): 373-9.
Ito M. 2012, Clin Calcium. 22 (3): 335-41.

Sino Biological Inc. liefert PTH / parathyroid hormone (aa 32-65) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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