GM2A Protein (His Tag)

18.04.2012

Produkt-Information von GM2A Protein

Synonym	GM2-AP, SAP-3
Protein Construction	A DNA sequence encoding the human GM2A (AAA35907.1) (Met 1- Ile 193) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von GM2A Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: GM2A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 22
Molecular Mass	The secreted recombinant human GM2A (pro form) comprises 180 amino acids and has a predicted molecular mass of 19.8 kDa. The apparent molecular mass of rh GM2A is approximately 25 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10%gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von GM2A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GM2A Protein

GM2A is a lipid transfer protein that stimulates the enzymatic processing of gangliosides, and also T-cell activation through lipid presentation. This protein binds molecules of ganglioside GM2, extracts them from membranes, and presents them to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. It is a member of ML domain family of proteins involved in innate immunity and lipid metabolism in the SMART database. GM2A Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. GM2A mutations are rarely reported, and the cases that are observed often occur with consanguineous parents or in genetically isolated populations. It is likely that most mutations of GM2A are fatal in the fetus in homozygotes and genetic compounds, and thus are never observed clinically.

References

Higashi K. et al., 2010, Biochem Biophys Res Commun. 402 (3): 571-6.
Higashi K. et al., 2011, FEBS Let. 585 (16): 2587-91.
Wright CS. et al., 2003, J Mol Biol. 331 (4): 951-64.

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