PS6K / RPS6KB1 Protein (GST Tag)

23.11.2012

Sino Biological Inc. liefert PS6K / RPS6KB1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat PS6K / RPS6KB1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/PS6K-RPS6KB1-Protein-g-9905.html

Produkt-Information von PS6K / RPS6KB1 Protein

Synonym	RPS6KB1, STK14A
Protein Construction	A DNA sequence encoding the human RPS6KB1 (P23443-Alpha I) (Met1-Leu525) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PS6K / RPS6KB1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PS6K protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human RPS6KB1 consists of 750 amino acids and predicts a molecular mass of 85.4 KDa. The apparent molecular mass of the protein is approximately 96 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, pH7.4, 10%gly, 1mM GSH. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PS6K / RPS6KB1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PS6K / RPS6KB1 Protein

RPS6KB1, also known as PS6K, is a serine/threonine-protein kinase. It is a member of the RSK (ribosomal s6 kinase) family. S6K1 contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. It plays a role in downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. S6K1 regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. It also mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. S6K1 may be one of many protein kinases involved in muscle building such as bony defects, delayed union, non-union fractures and osteoporosis.

References

Panasyuk. et al., 2006, J Biol Chem. 281 (42): 31188-201.
Carnevalli L. et al., 2010, Dev Cell. 18 (5): 763-74.
Grove JR. et al., 1991, Mol Cell Biol. 11 (11): 5541-50.