Vimentin / VIM Protein (His Tag)

23.11.2012

Sino Biological Inc. liefert Vimentin / VIM Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Vimentin / VIM Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Vimentin-VIM-Protein-g-9904.html

Produkt-Information von Vimentin / VIM Protein

Synonym	VIM
Protein Construction	A DNA sequence encoding the human VIM ( Met 1- Glu466) (P08670) was expressed, with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Vimentin / VIM Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: Vimentin protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human VIM consists of 476 amino acids and predicts a molecular mass of 55 KDa. The apparent molecular mass of the protein is approximately 56 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 40% acetonitrile +0.1%TFA. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Vimentin / VIM Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Vimentin / VIM Protein

Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. It exists around the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. The basic subunit of vimentin assembly is composed by two vimentin monomers. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. It also controls the transport of low-density lipoprotein and can be used as a sarcoma tumor marker to identify mesenchyme.

References

Russell RL. et al., 2001, J Biol Chem. 276 (16): 13302-7.
Moinova. et al., 2012, Cancer Epidemiology Biomarkers Prev. 21 (4): 594-600.
Leader M. et al., 1987, Histopathology. 11 (1): 63-72.