PRKAR1A / PKR1 / PRKAR1 Protein (His Tag)

19.06.2013

Sino Biological Inc. liefert PRKAR1A / PKR1 / PRKAR1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PRKAR1A / PKR1 / PRKAR1 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/PRKAR1A-PRKAR1-PKR1-Protein-Antibody-a-6228.html

Produkt-Information von PRKAR1A / PKR1 / PRKAR1 Protein

Synonym	PRKAR1A, PKR1, PRKAR1, TSE1
Protein Construction	A DNA sequence encoding the human PRKAR1A (P10644) (Met1-Val381) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von PRKAR1A / PKR1 / PRKAR1 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: PRKAR1A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PRKAR1A consists of 391 amino acids and predicts a molecular mass of 44.3 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PRKAR1A / PKR1 / PRKAR1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PRKAR1A / PKR1 / PRKAR1 Protein

PRKAR1A, also known as PRKAR1 and PKR1, is one of the regulatory subunits of cAMP-dependent protein kinase A (PKA). PKA can be activated by cAMP. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating PKA, which transduces the signal throughphosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR1A was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids Three alternatively spliced transcript variants encoding the same protein have been observed.

References

Huang L J. et al., 1997, Proc Natl Acad Sci. 94 (21): 11184-9.
Herberg F W. et al., 2000, J Mol Biol. 298 (2): 329-39.
Scambler P. et al., 1987, Am J Hum Genet. 41 (5): 925-32.