GADD45G / CR6 Protein (Native)

19.06.2013

Sino Biological Inc. liefert GADD45G / CR6 Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GADD45G / CR6 Protein (Native) lesen Sie bitte:http://www.sinobiological.com/GADD45G-CR6-Protein-Anibody-a-6230.html

Produkt-Information von GADD45G / CR6 Protein

Synonym	GADD45G, CR6, DDIT2
Protein Construction	A DNA sequence encoding human GADD45G (O95257) (Met1-Glu159) was expressed.
Source	Human
Expression Host	E.coli

QC Testing von GADD45G / CR6 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: GADD45G protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human GADD45G consists of 159 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 17 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GADD45G / CR6 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GADD45G / CR6 Protein

GADD45G, also known as CR6, is part of the nuclear proteins to interact with various proteins whose transcript levels are raised after stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45G reacts to environmental stresses by mediating activation of the p38/JNK pathway which is mediated through their protein binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. GADD45G acts as a new-age tumor suppressor however is being frequently inactivated epigenetically in multiple tumors. GADD45G mRNA expression is down-regulated in hepatocellular carcinoma. GADD45G causes cell cycle arrest at G2/M transition when transfected into Hep-G2 cells. GADD45G induction by androgens involves new protein synthesis. Overexpression of GADD45G inhibits cell growth and causes morphological modifications in prostate cell lines thus GADD45G takes part in differentiation induction by androgens.

References

Takekawa M. et al., 1998, Cell. 95 (4): 521-30.
Suzuki M. et al., 1999, J Hum Genet. 44 (5): 300-3.
Azam N. et al., 2001, J Biol Chem. 276 (4): 2766-74.