PRC1 Protein (His Tag)

28.03.2012

Produkt-Information von PRC1 Protein

Synonym	ASE1
Protein Construction	A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PRC1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PRC1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PRC1 consists of 639 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0, 20%gly, 3mM DTT Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

PRC1 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PRC1 Protein

PRC1(protein regulator of cytokinesis 1,N-Histidine-tagged) is involved in cytokinesis. It is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. PRC1 is e sential for controlling the spatiotemporal formation of the midzone. It is required for KIF14 localization to the central spindle and midbody. PRC1 has been shown to interact with TRIM37. It stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1.

References

Jiang W. et al., 1999, Mol Cell. 2 (6): 877-85.
Rual. et al., 2005, Nature (England). 437 (7062): 1173-8.
Mollinari C. et al., 2002, J Cell Biol. 157 (7): 1175-86.

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