ALPI / IAP Protein

28.03.2012

Produkt-Information von ALPI / IAP Protein

Synonym	IAP
Protein Construction	A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1- Asp 503) ,without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von ALPI / IAP Protein

Purity	> 83 % as determined by SDS-PAGE	SDS-PAGE: ALPI / IAP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 20
Molecular Mass	The secreted recombinant human ALPI/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 725 amino acids and has a predicted molecular mass of 79.5 kDa. The apparent molecular mass of rhALPI /Fc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

ALPI / IAP Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ALPI / IAP Protein

Alkaline phosphatase, intestinal also known as ALPI is digestive brush-border enzyme which is upregulated during small intestinal epithelial cell differentiation. ALPI is also referred to as IAP and it is released into the lumen after a high-fat diet. ALPI / IAP is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. Lysophosphatidylcholine (LysoPC) releases IAP into the apical medium more than other intestinal brush-border enzymes, sucrose, aminopeptidase N, and lactase, without comparable lactate dehydrogenase release or cell injury. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver / bone / kidney (tissue non-specific). Intestinal ALP shows 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver / bone / kidney ALP.

References

Nakano T. et al., 2009, Am J Physiol Gastrointest Liver Physiol. 297 (1): 207-14.
Henthorn PS. et al., 1987, Proc Natl Acad Sci. 84 (5): 1234-8.
Oetting WS. et al., 2003, Pigment Cell Res. 16 (3): 307-11.

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