PPP3R1 Protein (His Tag)

11.10.2012

Produkt-Information von PPP3R1 Protein

Synonym	PPP3R1, CNA2, CNB
Protein Construction	A DNA sequence encoding the human PPP3R1 (P63098)(Gly2-Val170) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PPP3R1 Protein

Purity	> 87% as determined by SDS-PAGE	SDS-PAGE: PPP3R1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PPP3R1 consists of 187 amino acids and has a calculated molecular mass of 21.4 kDa. The recombinant protein migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 50mM Tris,100mM NaCl,pH 8.0, 10%glycerol, 2mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PPP3R1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PPP3R1 Protein

PPP3R1 is a member of the calcineurin regulatory subunit family. Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). PPP3R1 interacts with (+)-pilocarpine, 2,4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway.

References

Feng B. et al., 1999, Biochemistry 38 (38): 12481-9.
Kawamura A. et al., 1995, J Biol Chem. 270 (26): 15463-6.
Wang MG. et al., 1997, Cytogenet Cell Genet. 72 (2-3): 236-41.

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