PPP3CA / Protein phosphatase 2B Protein (His Tag)

11.10.2012

Produkt-Information von PPP3CA / Protein phosphatase 2B Protein

Synonym	PPP3CA, CALNA, CNA
Protein Construction	A DNA sequence encoding the human PPP3CA (Q08209-1)(Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PPP3CA / Protein phosphatase 2B Protein

Purity	> 94% as determined by SDS-PAGE	SDS-PAGE: PPP3CA protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PPP3CA consists of 538 amino acids and has a calculated molecular mass of 60.8 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0, 10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PPP3CA / Protein phosphatase 2B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PPP3CA / Protein phosphatase 2B Protein

PPP3CA, also known as protein phosphatase 2B, belongs to the PPP phosphatase family, PP-2B subfamily. It is a subunit of protein phosphatase 2B (PP2B). PP2B comprises two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. PPP3CA is the alpha catalytic subunit. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.

References

Frey N. et al., 2002, Journal of Biological Chemistry. 277 (16): 13998-4004.
Frey N. et al., 2000, Proceedings of the National Academy of Sciences. 97 (26): 14632-7.
Crabtree G R. et al., 1999, Cell. 96 (5): 611-4.

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