PLEK / Pleckstrin Protein (His Tag)

24.04.2013

Sino Biological Inc. liefert PLEK / Pleckstrin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PLEK / Pleckstrin Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Pleckstrin-PLEK-p47-Protein-Antibody-a-5873.html

Produkt-Information von PLEK / Pleckstrin Protein

Synonym	PLEK, P47
Protein Construction	A DNA sequence encoding the mature form of human PLEK (XP_004029394.1) (Met1-Lys350) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PLEK / Pleckstrin Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: PLEK protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PLEK consists of 365 amino acids and predicts a molecular mass of 41.9 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PLEK / Pleckstrin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PLEK / Pleckstrin Protein

Pleckstrin is a protein found in platelets. Pleckstrin is the source of the name pleckstrin homology domain. Pleckstrin homology domain (PH domain) is a protein domain of approximately 120 amino acids that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton. This domain can bind Phosphatidylinositol lipids within biological membranes (such as Phosphatidylinositol (3,4,5)-trisphosphate and phosphatidylinositol (4,5)-bisphosphate), and proteins such as the βγ-subunits of heterotrimeric G proteins, and protein kinase C. Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways.

References

Ingley E. et al., 1994, J Cell Biochem. 56 (4): 436-43.
Gibson TJ. et al., 1994, Trends Biochem Sci. 19 (9): 349-53.
Haslam RJ. et al., 1993, Nature. 363 (6427): 309-10.

Pleckstrin (PLEK, p47) related areas, pathways, and other information