PGC / pepsinogen C Protein (His Tag)

13.03.2013

Sino Biological Inc. liefert PGC / pepsinogen C Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PGC / pepsinogen C Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PGC-pepsinogen-C-Protein-g-10603.html

Produkt-Information von PGC / pepsinogen C Protein

Synonym	PGC
Protein Construction	A DNA sequence encoding the mature form of human PGC (P20142-1) (Ile153-Ile239) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PGC / pepsinogen C Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: PGC protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PGC consists of 102 amino acids and predicts a molecular mass of 10.9 KDa. It migrates as an approximately 10 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 0.4M sucrose, 1mM EDTA, 50mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PGC / pepsinogen C Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PGC / pepsinogen C Protein

Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. Upon ingestion of food, each of the zymogens is released into the gastric lumen and undergoes conversion into active enzyme in the acidic gastric juice. This activation reaction is nitiated by the disruption of electrostatic interactions between the prosegment and the active enzyme moiety at low pH values. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.

References

Richter C. et al., 1998, Biochem J. 1 (335): 481-90.
Westerveld BD. et al., 1987, Gastroenterology. 93 (4): 774-8.
Ichinose M. et al., 1991, Jpn J Cancer Res. 82 (6): 686-92.