Dehydropeptidase-I / DPEP1 Protein (His Tag)

14.03.2013

Sino Biological Inc. liefert Dehydropeptidase-I / DPEP1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Dehydropeptidase-I / DPEP1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Dehydropeptidase-I-DPEP1-Protein-g-10604.html

Produkt-Information von Dehydropeptidase-I / DPEP1 Protein

Synonym	DPEP1, MDP, RDP
Protein Construction	A DNA sequence encoding the human DPEP1 (P16444) (Met1-Ser385) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

Dehydropeptidase-I / DPEP1 Protein QC Testing

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: DPEP1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 17
Molecular Mass	The recombinant human DPEP1 consists of 380 amino acids and predicts a molecular mass of 42.5 KDa. It migrates as an approximately 44-47 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Dehydropeptidase-I / DPEP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Dehydropeptidase-I / DPEP1 Protein

Dehydropeptidase-I, also known as DPEP1, is a kidney membrane enzyme. Its expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. The overexpression of DPEP1 suppressed tumor cells invasiveness and increased sensitivity to chemotherapeutic agent Gemcitabine. Growth factor EGF treatment decreased DPEP1 expression. Dehydropeptidase-I may be a candidate target in PDAC for designing improved treatments. It uses zinc as a cofactor and acts as a disulfide-linked homodimer.

References

Toiyama Y. et al., 2011, J Gastroenterol. 46 (2): 153-63.
Zhang G. et al., 2012, PLoS One. 7 (2): e31507.
Okamoto T. et al., 2011, Mod Pathol. 24 (2): 267-76.