Cynomolgus SELL / Selectin L / L-selectin Protein (His Tag)

13.03.2013

Sino Biological Inc. liefert Cynomolgus SELL / Selectin L / L-selectin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus SELL / Selectin L / L-selectin Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SELL-Selectin-L-L-selectin-Protein-g-10587.html

Produkt-Information von SELL / Selectin L / L-selectin Protein

Synonym	SELL
Protein Construction	A DNA sequence encoding the cynomolgus SELL (Met1- Asn332) was expressed with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von SELL / Selectin L / L-selectin Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: SELL protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 29
Molecular Mass	The recombinant cynomolgus SELL comprises 315 amino acids and has a calculated molecular mass of 35.6 KDa. The apparent molecular mass of it is approximately 52-63 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SELL / Selectin L / L-selectin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SELL / Selectin L / L-selectin Protein

L-selectin ( SELL ), also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups and are involved in the regulation of leukocyte traffic. Selectins are composed of an N-terminal C-type lectin domain, a single EGF domain, a variable number of short consensus repeat (SCR) domains, a transmembrane region and a cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs.

References

Kansas GS. 1992, APMIS. 100 (4): 287-93.
Stamenkovic I. 1995, Curr Opin Hematol. 2 (1): 68-75.
Brenner B. et al., 1997, Proc Natl Acad Sci. 93 (26): 15376-81.