Periostin / POSTN / OSF-2 Antikörper (Antigen Affinität reinigt)

02.07.2012

Periostin / POSTN / OSF-2 Antikörper

Immunogen	Recombinant Human Periostin / POSTN / OSF-2 protein (Catalog#50671-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human Periostin / POSTN / OSF-2 (rh Periostin / POSTN / OSF-2; Catalog#10299-H08H; NP_001129407.1; Met 1-Gln 781). Periostin / POSTN / OSF-2 specific IgG was purified by Human Periostin / POSTN / OSF-2 affinity chromatography.

Periostin / POSTN / OSF-2 Antibody Usage Guide

Specificity

Human Periostin / POSTN / OSF-2

Immunochemistry

IHC-P 0.1-2 μg/mL

POSTN-colon immunohistochemistry POSTN-colon-carcinoma immunohistochemistry

Immunochemical staining of human POSTN in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

Immunochemical staining of human POSTN in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

POSTN-stomach immunohistochemistry POSTN-breast-carcinoma immunohistochemistry

Immunochemical staining of human POSTN in human stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

Immunochemical staining of human POSTN in human breast carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

Western blot

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human POSTN in WB. Using a DAB detection system, the detection limit for Human POSTN is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.

Direct ELISA

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human POSTN. The detection limit for Human POSTN is approximately 0.00975 ng/well.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von Periostin / POSTN / OSF-2 Antikörper

Periostin (POSTN), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.

References

Kudo,Y. et al., 2007, Histol Histopathol. 22 (10):1167-1174.
Li, J.S. et al., 2007, World J Gastroenterol. 13 (39): 5261-5266.
Oku, E. et al., 2008, Int J Hematol. 88 (1): 57-63.
Hamilton, D.W. et al., 2008, J Cell Commun Signal. 2 (1-2):9-17.
Puglisi, F.J et al., 2008, Clin Pathol. 61 (4): 494-498.
Conway, S. J. et al., 2008, Curr Genomics. 9 (8): 548-555.

Sino Biological Inc. liefert Periostin / POSTN / OSF-2 Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Periostin / POSTN / OSF-2 Antikörper (Antigen Affinität reinigt) lesen Sie bitte: http://www.sinobiological.com/Periostin-POSTN-OSF-2-Antibody-g-9111.html

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
POSTN/OSF-2	Human	Periostin/POSTN/OSF-2 Protein, Recombinant	10299-H08H
POSTN/OSF-2	Mouse	Periostin/POSTN/OSF-2 Protein, Recombinant	50450-M08H

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human POSTN/OSF-2	WB, ELISA, IHC-P	Periostin / POSTN / OSF-2 Antibody (Antigen Affinity Purified)	10299-RP02
Mouse POSTN/OSF-2	WB, ELISA	Periostin / POSTN Antibody	50450-R031

Periostin / POSTN / OSF-2 Antikörper (Antigen Affinität reinigt)