PDE3A Protein (GST Tag)

16.10.2012

Produkt-Information von PDE3A Protein

Synonym	PDE3A
Protein Construction	A DNA sequence encoding the human PDE3A (Q14432)(Lys669-Gln1141) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PDE3A Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PDE3A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PDE3A /GST chimera consists of 710 amino acids and has a calculated molecular mass of 81.7 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4,10% gly, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PDE3A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PDE3A Protein

PDE3A is a member of the phosphodiesterase family. Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. These enzymes hydrolyze some nucleoside 3',5'-cyclic phosphate to some nucleoside 5'-phosphate. PDE3A has high affinity for both cAMP and cGMP and shows competitive inhibition of the cAMP hydrolytic activity by cGMP. It plays a critical role in regulating intracellular levels of cAMP and cGMP. Deletion of the N-terminus of PDE3A enhances the hydrolysis of cGMP relative to cAMP suggesting that the role of divergent N-termini of various PDEs could be to exert substrate specificity. Stimulation of platelets with thrombin increases PDE3A activity via Akt signaling pathway and this increase can be diminished by dephosphorylation of PDE3A by protein phosphatase 1. PDE3A has been implicated in cardiovascular function and fertility. The PDE3A assay Kit is designed for identification of PDE3A inhibitors using fluorescence polarization.

References

Penmatsa H. et al., 2010, Mol Biol Cell. 21(6):1097-110.
Zhang W. et al., 2007, Blood. 110(5):1475-82.
Degerman E. et al., 1997, J Biol Chem. 272(11):6823-6.

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