PD-L1 / B7-H1 / CD274 Antikörper

02.07.2012

Produkt-Information von PD-L1 / B7-H1 / CD274 Antikörper

Immunogen	Recombinant Human PD-L1 / B7-H1 / CD274 protein (Catalog#10084-H08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	015
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L1 / B7-H1 / CD274 (rh PD-L1 / B7-H1 / CD274; Catalog#10084-H08H; NP_054862.1; Met 1-Thr 239).

Usage Guide von PD-L1 / B7-H1 / CD274 Antikörper

Specificity

Human PD-L1 / B7-H1 / CD274

No cross-reactivity in ELISA with

Human B7-H1

Human B7-1

Human B7-2

Mouse B7-H1

Immunochemistry

IHC-P: 5-15 μg/mL

B7H1-placenta immunohistochemistry

Immunochemical staining of human B7H1 in human placenta with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human B7H1 in WB.

Direct ELISA

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human B7H1.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von PD-L1 / B7-H1 / CD274 Antikörper

Related Areas:

Immunology>>Adaptive Immunity>>Costimulation & Costimulatory Molecule>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Other>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Other>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Natural Killer (NK) Cell CD Antigen>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Other>>PD-L1/B7-H1/CD274

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
PD-L1/B7-H1/CD274	Human	PD-L1/B7-H1/CD274/Fc Protein, Recombinant	10084-H02H
PD-L1/B7-H1/CD274	Human	PD-L1/B7-H1/CD274 Protein, Recombinant	10084-H08H
PD-L1/B7-H1/CD274	Mouse	PD-L1/B7-H1/CD274/Fc Protein, Recombinant	50010-M03H
PD-L1/B7-H1/CD274	Mouse	PD-L1/B7-H1/CD274 Protein, Recombinant	50010-M08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Monoclonal Antibody	10084-R001
Human PD-L1/B7-H1/CD274	WB, ELISA, IHC-P	PD-L1 / B7-H1 / CD274 Antibody	10084-R015
Human PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody	10084-RP01
Human PD-L1/B7-H1/CD274	WB, ELISA	PD-L1 / B7-H1 / CD274 Antibody (Antigen Affinity Purified)	10084-RP02
Mouse PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody	50010-RP01
Mouse PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50010-RP02

Hintergrund von PD-L1 / B7-H1 / CD274 Antikörper

B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between B7-H1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. As a costimulator, B7-H1 induces T-cell proliferation and preferentially the production of interleukin 10 and interferon gamma. Conversely, by binding to programmed death 1 receptors (PD-1) on activated T-cells and B-cells, B7-H1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, B7-H1 leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. B7-H1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

References

Dong, H. et al., 1999, Nat. Med. 5: 1365-1369.
Freeman, G.J. et al., 2000, J. Exp. Med. 192:1027-103.
Dong, H., et al., 2002, Nat. Med. 8: 793-800.
Tamura, H. et al., 2003, Int. J. Hematol. 78: 321-328.
Trabattoni, D. et al., 2003, Blood. 101: 2514-2520.

Sino Biological Inc. liefert PD-L1 / B7-H1 / CD274 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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