Osteonectin / SPOCK1 (aa 21-429) Protein (His Tag)

03.04.2013

Sino Biological Inc. liefert Osteonectin / SPOCK1 (aa 21-429) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Osteonectin / SPOCK1 (aa 21-429) Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SPOCK1-Testican-1-Protein-Antibody-a-5762.html

Produkt-Information von Osteonectin / SPOCK1 Protein

Synonym	SPOCK1, SPOCK, TIC1, TICN1
Protein Construction	A DNA sequence encoding the human SPOCK1 (Q08629) ( Met 1-Trp439) was expressed with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Osteonectin / SPOCK1 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: Osteonectin protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Arg 22
Molecular Mass	The secreted recombinant human SPOCK1 consists of 428 amino acids and predicts a molecular mass of 48.4 KDa. The apparent molecular mass of the protein is approximately 53 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Osteonectin / SPOCK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Osteonectin / SPOCK1 Protein

Osteonectin, also known as SPOCK1, is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. They are characterized structurally by an N-terminal testican-specific domain, a follistatin-like region, a calcium-binding domain, a thyroglobulin-like domain, and an acidic C-terminal domain with two putative glycosaminoglycan attachment sites. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Osteonectin contains 1 Kazal-like domain and 1 thyroglobulin type-1 domain. Up to now, little is known about osteonectin’s function. It may play a role in cell-cell and cell-matrix interactions. Osteonectin also may contribute to various neuronal mechanisms in the central nervous system.

References

Marr HS. et al., 1998, Endothelium. 5 (3): 209-19.
Bonnet F. et al., 1996, J Biol Chem. 271 (8): 4373-80.
Perin JP. et al., 1993, Eur J Biochem. 214 (1): 347-50.
Roussel G. et al., 1997, C R Seances Soc Biol Fil. 191 (1): 127-33.

SPOCK1 (Testican 1) related areas, pathways, and other information