Cynomolgus PDCD1LG2 / B7-DC / PD-L2 Protein

03.04.2013

Sino Biological Inc. liefert Cynomolgus PDCD1LG2 / B7-DC / PD-L2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus PDCD1LG2 / B7-DC / PD-L2 Protein lesen Sie bitte: http://www.sinobiological.com/B7-DC-PD-L2-Protein-Antibody-a-561.html

Produkt-Information von PDCD1LG2 / B7-DC / PD-L2 Protein

Synonym	PDCD1LG2
Protein Construction	A DNA sequence encoding the cynomolgus PDCD1LG2(A4GW30) (Met1-Pro219) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von PDCD1LG2 / B7-DC / PD-L2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PDCD1LG2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 20
Molecular Mass	The recombinant cynomolgus PDCD1LG2 is a disulfide-linked homodimer. The reduced monomer comprises 441 amino acids and has a calculated molecular mass of 49.6 KDa. The apparent molecular mass of the protein is approximately 66 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PDCD1LG2 / B7-DC / PD-L2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PDCD1LG2 / B7-DC / PD-L2 Protein

Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is the ligands for programmed death-1 (PD-1), which is a member of the CD28/B7 family and has been implicated in peripheral tolerance. CD28/B7 signals have been shown to have the capacity to regulate T cell activation and participate in regulating the development of rheumatoid arthritis (RA). B7-DC fails to bind the B7-1/2 receptors CD28 and cytotoxic T lymphocyte–associated antigen (CTLA)-4, but does bind PD-1, a receptor for B7-H1/PD-L1. B7-DC costimulates T cell proliferation more efficiently than B7-1 and induces a distinct pattern of lymphokine secretion. In particular, B7-DC strongly costimulates interferon γ but not interleukin (IL)-4 or IL-10 production from isolated naive T cells. On the cellular level, all of them were found on cell membrane and in cytoplasma. The expression of B7-DC and B7-H3 was major on capillaries, synovicytes and infiltrated inflammatory cells in the lining layer, from RA patients. B7-DC expression was scarcely expressed on dendritic cells in naive mice, but was up-regulated after allergen challenge, although the up-regulation of B7-DC expression on macrophages was minimal.

References

Greenwald RJ. et al., 2005, Annu Rev Immunol. 23: 515-48.
Matsumoto K. et al., 2004, The Journal of Immunology. 172: 2530-41.
Tseng SY. et al., 2001, JEM. 193 (7): 839-46.