NMNAT1 / NMNAT Protein (His Tag)

10.04.2013

Sino Biological Inc. liefert NMNAT1 / NMNAT Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NMNAT1 / NMNAT Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/NMNAT1-Protein-Antibody-a-5774.html

Produkt-Information von NMNAT1 / NMNAT Protein

Synonym	NMNAT1, NMNAT
Protein Construction	A DNA sequence encoding the human NMNAT1 (Q9HAN9)( Met 1-Thr279) was expressed with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von NMNAT1 / NMNAT Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: NMNAT1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human NMNAT1 consists of 289 amino acids and predicts a molecular mass of 33.3 KDa. The apparent molecular mass of the protein is approximately 34 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NMNAT1 / NMNAT Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NMNAT1 / NMNAT Protein

NMNAT, also known as NMNAT1, is a member of the Nicotinamide-nucleotide adenylyltransferases. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. NMNAT appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.

References

Sugano S. et al., 1994, Gene. 138 (1-2): 171-4.
Saccucci F. et al., 2001, J Biol Chem. 276 (1): 406-12.
Hennig K. et al., 2001, FEBS Lett. 492 (1-2): 95-100.

NMNAT1 related areas, pathways, and other information