OPTN / Optineurin Protein (GST Tag)

09.05.2013

Sino Biological Inc. liefert OPTN / Optineurin Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über OPTN / Optineurin Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/Optineurin-OPTN-Protein-Antibody-a-5919.html

Produkt-Information von OPTN / Optineurin Protein

Synonym	OPTN, FIP2, GLC1E, HIP7, HYPL, NRP
Protein Construction	A DNA sequence encoding the human OPTN (NP_068815.2) (Met1-Ile577) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von OPTN / Optineurin Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE OPTN protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human OPTN chimera consists of 814 amino acids and has a calculated molecular mass of 93.7 kDa. The recombinant protein migrates approximately 94 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von OPTN / Optineurin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von OPTN / Optineurin Protein

Optineurin gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

References

Rezaie T. et al., 2002, Science. 295 (5557): 1077-9.
Li Y. et al., 1998, Mol Cell Biol. 18 (3): 1601-10.
Skarnes Wc RB. et al., 2011, Nature. 474 (7351): 337-42.