OPALIN Protein

Sino Biological Inc. liefert OPALIN Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über OPALIN Protein lesen Sie bitte: http://www.sinobiological.com/Opalin-TMEM10-Protein-Antibody-a-5750.html

Produkt-Information von OPALIN Protein

QC Testing von OPALIN Protein

Purity	(80.7+11.2)% as determined by SDS-PAGE	SDS-PAGE: OPALIN protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp
Molecular Mass	The recombinant human OPALIN/mFc  comprises 327 amino acids and has a predicted molecular mass of 37.4 kDa. The apparent molecular mass of the monomer is approximately 44 and 33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von OPALIN Protein

Verwandte Produkte & Themen von OPALIN Protein

Beschreibung von OPALIN Protein

Opalin, or oligodendrocytic myelin paranodal and inner loop protein, is a transmembrane protein detected specifically in mammalian oligodendrocytes, and may play significant role in oligodendrocyte differentiation and myelination.Opalin has binding sites for Myt1 and cAMP-response element binding protein (CREB). Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. Thus LIF, cAMP signaling cascades and Myt1 may play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer. Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. Site-directed mutations at the glycan sites impaired the cell surface localization of Opalin. In addition to the somata and processes of oligodendrocytes, Opalin immunoreactivity was observed in myelinated axons in a spiral fashion, and was concentrated in the paranodal loop region. Immunogold electron microscopy demonstrated that Opalin was localized at particular sites in the paranodal loop membrane. These results suggest a role for highly sialylglycosylated Opalin in an intermembranous function of the myelin paranodal loops in the central nervous system.

References

1. Aruga J. et al., 2007, J Neurochem. 102 (5): 1533-47.
2. Kippert A. et al., 2008, BMC Neurosci. 9: 40.
3. Yoshikawa F. et al., 2008, J Biol Chem. 83 (30): 20830-40.
4. Golan N. et al., 2008, Glia. 56 (11): 1176-86.

Opalin (TMEM10) related areas, pathways, and other information