NT5C3 Protein (Native)

11.09.2012

Produkt-Information von NT5C3 Protein

Synonym	P5N1, UMPH1, NT5C3
Protein Construction	A DNA sequence encoding the mature form of human NT5C3 (NP_001002010) (Met 12- Leu 297) was expressed.
Source	Human
Expression Host	E.coli

QC Testing von NT5C3 Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: NT5C3 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met 12
Molecular Mass	The recombinant human NT5C3 consists of 286 amino acids and predicts a molecular mass of 32.7KDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von NT5C3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NT5C3 Protein

NT5C3 is a member of the pyrimidine 5'-nucleotidase(P5N) family. The pyrimidine 5'-nucleotidase (P5N) includes type I and type II isozymes, which catalyze the dephosphorylation of the pyrimidine 5'-monophosphates UMP and CMP to the corresponding nucleosides. NT5C3 gene encodes the type I P5N isozyme and a gene on chromosome 17 encodes the type II P5N isozyme. Both genes are members of the nucleotidase gene family. Mutations in this gene are the main cause of P5N deficiency, which results in an autosomal recessive hemolytic anemia. It was reported that this gene had two pseudogenes on chromosomes 4 and 7, respectively, but the pseudogene on chromosome 7 is not verified. NT5C3 functions both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency which is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte.

References

Hillier LD. et al., 1997, Genome Res. 6 (9): 807-28.
Jordheim LP. et al., 2008, Drug Metab Dispos. 36 (12): 2419-23.
Aksoy P. et al., 2009, Pharmacogenet Genomics. 19 (8): 567-76.

Sino Biological Inc. liefert NT5C3 Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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