NQO1 / DT-diaphorase Protein (His Tag)

10.04.2013

Sino Biological Inc. liefert NQO1 / DT-diaphorase Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NQO1 / DT-diaphorase Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/NQO1-Protein-Antibody-a-5768.html

Protein-Information von NQO1 / DT-diaphorase Protein

Synonym	NQO1
Protein Construction	A DNA sequence encoding the mature form of human NQO1 (P15559-1) (Met1-Lys274) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von NQO1 / DT-diaphorase Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: NQO1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human NQO1 consists of 290 amino acids and predicts a molecular mass of 33 KDa. It migrates as an approximately 33 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NQO1 / DT-diaphorase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NQO1 / DT-diaphorase Protein

NQO1 gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. NQO1 forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in NQO1 gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recent pharmacological research suggests feasibility of genotype-directed redox chemotherapeutic intervention targeting NQO1 breast cancer, a common missense genotype encoding a functionally impaired NQO1 protein.

References

Ross David. et al., 2002, J Biol Chem. 277 (16): 14060-7.
Cabello CM. et al., 2010, Free Radic Res. 45 (3): 276-92.
Adesnik M. et al., 1988, J Biol Chem. 263 (27): 13572-8.

NQO1 related areas, pathways, and other information