CALU / Calumenin Protein (His Tag)

08.01.2013

Sino Biological Inc. liefert CALU / Calumenin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CALU / Calumenin Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CALU-Calumenin-Protein-g-10312.html

Produkt-Information von CALU / Calumenin Protein

Synonym	CALU
Protein Construction	A DNA sequence encoding the human CALU (O43852-1) (Met1-Arg311) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CALU / Calumenin Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: CALU protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 20
Molecular Mass	The recombinant human CALU consists of 303 amino acids and predicts a molecular mass of 35.9 KDa. It migrates as an approximately 46-52 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CALU / Calumenin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CALU / Calumenin Protein

Calumenin is a member of the CREC family. CERC is an acronym for its family members Cab-45, reticulocalbin, Erc-55 (RCN2), and calumenin. Calumenin is a calcium-binding protein localized in the endoplasmic reticulum (ER) and is involved in such ER functions as protein folding and sorting. Calumenin contains 6 EF-hand domains and is expressed in skeletal muscle (at protein level). Calumenin is Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. It seems to inhibit gamma-carboxylase GGCX. Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum.

References

Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.
Vorum H. et al., 2000, FEBS Lett. 465 (2-3): 129-34.
Vorum H. et al., 1999, Exp Cell Res. 248 (2): 473-81.