NEIL1 Protein (His Tag)

04.05.2012

Produkt-Information von NEIL1 Protein

Synonym	FPG1, NEI1, hFPG1
Protein Construction	A DNA sequence encoding the human NEIL1 (AAH10876.1) (Met 1-Ser 390) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von NEIL1 Protein

Purity	> 84 % as determined by SDS-PAGE	SDS-PAGE: NEIL1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met 1
Molecular Mass	The recombinant human NEIL1 comprises 400 amino acids and migrates as an approximately 45 kDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 50mM Tris,150mM NaCl; pH8.0 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von NEIL1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NEIL1 Protein

NEIL1 belongs to the DNA glycosylases family. DNA glycosylases are enzymes that participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. They initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 also has marginal activity towards 8-oxoguanine and AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.

References

Zhang QM. et al., 2005, DNA Repair. 4 (1): 71-9.
Mokkapati SK. et al., 2004, Biochemistry. 43 (36): 11596-604.
Shinmura K. et al., 2005, Carcinogenesis. 25 (12): 2311-7.
Doublié S. et al., 2004, Proc Natl Acad. 101 (28): 10284-9.

Sino Biological Inc. liefert NEIL1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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