ALDH4A1 Protein (GST Tag)

04.05.2012

Produkt-Information von ALDH4A1 Protein

Synonym	RP11-128M10.1, ALDH4, P5CD, P5CDh
Protein Construction	A DNA sequence encoding the mature form of human ALDH4A1 (AAH07581.1) (Lys 25-Gln 563) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ALDH4A1 Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: ALDH4A1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human ALDH4A1/GST chimera consists of 776 amino acids and has a calculated molecular mass of 86.8 KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.5, 10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ALDH4A1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ALDH4A1 Protein

ALDH4A1 belongs to the aldehyde dehydrogenase family. It is a mitochondrial matrix homodimer (~61.7 kDa subunits) and catalyzes the second step in the proline degradation pathway - the irreversible conversion of Δ1-pyrroline-5-carboxylate (P5C) derived either from proline or ornithine to glutamate. This reaction is a necessary step connecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic. Recent studies have shown that ALDH4A1 is transcriptionally induced by p53 and potentially may have a protective role during cellular stress. Deficiency of ALDH4A1 is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.

References

Goodman SI. et al., 1974, Biochemical medicine. 10 (4): 329-36.
Maruyama K. et al., 1994, Gene. 138 (1-2): 171-4.
Vasiliou V. et al., 2005, Hum Genomics. 2 (2): 138-43.

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