NCF2 / NCF-2 / P67phox Protein

21.06.2013

Sino Biological Inc. liefert NCF2 / NCF-2 / P67phox Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NCF2 / NCF-2 / P67phox Protein lesen Sie bitte:http://www.sinobiological.com/NCF-2-NCF2-p67phox-Protein-Antibody-a-6221.html

Produkt-Information von NCF2 / NCF-2 / P67phox Protein

Synonym	NCF2
Protein Construction	A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von NCF2 / NCF-2 / P67phox Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: NCF2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The secreted recombinant human NCF2 consists of 528 amino acids and predicts a molecular mass of 59.9 KDa. The apparent molecular mass of the protein is approximately 60 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NCF2 / NCF-2 / P67phox Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NCF2 / NCF-2 / P67phox Protein

NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

References

Wientjes FB. et al., 1996, Semin Cell Biol. 6 (6): 357-65.
DeLeo FR. et al., 1997, J Leukoc Biol. 60 (6): 677-91.
Dorseuil O. et al., 1997, C R Seances Soc Biol Fil. 191 (2): 237-46.