GALE / UDP galactose-4-epimerase Protein (His Tag)

21.06.2013

Sino Biological Inc. liefert GALE / UDP galactose-4-epimerase Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GALE / UDP galactose-4-epimerase Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/UDP-galactose-4'-epimerase-GALE-Protein-Antibody-a-6232.html

Produkt-Information von GALE / UDP galactose-4-epimerase Protein

Synonym	GALE
Protein Construction	A DNA sequence encoding the mature form of human GALE (Q14376) (Met1-Ala348) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von GALE / UDP galactose-4-epimerase Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: GALE protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human GALE consists of 363 amino acids and predicts a molecular mass of 40.1 KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM HEPES, 150mM NaCl, 10% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GALE / UDP galactose-4-epimerase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GALE / UDP galactose-4-epimerase Protein

UDP galactose-4'-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4'-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

References

Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Lee KA. et al., 2011,. J Biol Chem. 286 (48): 41530-8.
McCorvie TJ. et al., 2012, Biochim Biophys Acta. 1822 (10): 1516-26.