NCF-2 / NCF2 / P67phox Protein (GST Tag)

08.06.2013

Sino Biological Inc. liefert NCF-2 / NCF2 / P67phox Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NCF-2 / NCF2 / P67phox Protein (GST Tag) lesen Sie bitte:http://www.sinobiological.com/NCF-2-NCF2-p67phox-Protein-Antibody-a-6221.html

Produkt-Information von NCF-2 / NCF2 / P67phox Protein

Synonym	NCF2, NOXA2, P67PHOX
Protein Construction	A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von NCF-2 / NCF2 / P67phox Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: NCF-2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human NCF2 /GST chimera consists of 763 amino acids and has a calculated molecular mass of 87.6 kDa. The recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NCF-2 / NCF2 / P67phox Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NCF-2 / NCF2 / P67phox Protein

NCF-2, also known as NCF2 and p67phox, is the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. NADPH oxidase plays an essential role in the immune system. It is primarily active in immune system cells called phagocytes. These cells catch and destroy foreign invaders such as bacteria and fungi. NADPH oxidase also participates in a chemical reaction that converts oxygen to a toxic molecule called superoxide. Superoxide is used to generate several other compounds, including hydrogen peroxide (a strong disinfectant) and hypochlorous acid (the active ingredient in bleach). These highly reactive, toxic substances are known as reactive oxygen species. Phagocytes use these substances to kill foreign invaders, preventing them from reproducing in the body and causing illness. Mutations in NCF-2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

References

Cunninghame Graham DS. et al., 2011, PLoS Genet. 7 (10): e1002341.
Jacob CO. et al., 2012,?Proc Natl Acad Sci. 109 (2): E59-67.
Martel C. et al., 2012,?J Clin Immunol. 32 (5): 942-58.