Biotinidase / Biotinase / BTD Protein (His Tag)

30.05.2013

Sino Biological Inc. liefert Biotinidase / Biotinase / BTD Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Biotinidase / Biotinase / BTD Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/Biotinase-BTD-Protein-Antibody-a-6201.html

Produkt-Information von Biotinidase / Biotinase / BTD Protein

Synonym	BTD
Protein Construction	A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von Biotinidase / Biotinase / BTD Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: Biotinidase protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 42
Molecular Mass	The recombinant human BTD comprises 513 amino acids and has a predicted molecular mass of 58.2 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Biotinidase / Biotinase / BTD Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Biotinidase / Biotinase / BTD Protein

Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

References

Pindolia K. et al. 2010, Human mutation. 31 (9): 983-91.
Dobrowolski SF. et al., 2003, Mol Genet Metab. 78 (2): 100-7.
McMahon RJ. 2002, Annu Rev Nutr. 22: 221-39.