MVK / Mevalonate kinase Protein (GST Tag)

10.12.2012

Sino Biological Inc. liefert MVK / Mevalonate kinase Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über MVK / Mevalonate kinase Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/MVK-Mevalonate kinase-Protein-g-9978.html

Produkt-Information von MVK / Mevalonate kinase Protein

Synonym :	MVK
Protein Construction:	A DNA sequence encoding the human MVK (Q03426) (Met1-Leu396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source:	Human
Expression Host:	Baculovirus-Insect cells

QC Testing von MVK / Mevalonate kinase Protein

Purity:	> 90% as determined by SDS-PAGE	SDS-PAGE: MVK protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Met
Molecular Mass:	The recombinant human MVK /GST chimera consists of 633 amino acids and has a calculated molecular mass of 70.2 kDa. The recombinant protein migrates approximately 47 kDa band in SDS-PAGE under reducing conditions.
Formulation:	Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM DTT, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MVK / Mevalonate kinase Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MVK / Mevalonate kinase Protein

Mevalonate kinase is a member of the GHMP kinase family, mevalonate kinase subfamily. It can be found in a wide variety of organisms from bacteria to mammals. Mevalonate kinase plays a role in the synthesis of isopentanyl pyrophosphate, a common intermediate for a number of pathways including cholesterol biosynthesis. It is also involved in mevalonate catabolism. Defects in mevalonate kinase can cause mevalonic aciduria. Defects in mevalonate kinase can also cause hyperimmunoglobulinemia D and periodic fever syndrome (HIDS). HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis.

References

Fu Z. et al., 2008, Biochemistry. 47 (12): 3715-24.
Houten SM. et al., 2000, Biochim Biophys Acta. 1529 (1-3): 19-32.
Schafer BL. et al., 1992, J Biol Chem. 267 (19): 13229-38.