CKMT1A Protein (His Tag)

10.12.2012

Sino Biological Inc. liefert CKMT1A Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CKMT1A Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CKMT1A-Protein-g-9979.html

Produkt-Information von CKMT1A Protein

Synonym	CKMT1A, CKMT
Protein Construction	A DNA sequence encoding human CKMT1A (P12532-1) (Ala40-His417) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CKMT1A Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CKMT1A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CKMT1A consists of 396 amino acids and predicts a molecular mass of 45.3 KDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CKMT1A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CKMT1A Protein

CKMT1A contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It is a member of the ATP:guanido phosphotransferase family. It reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. CKMT1A also can be taken as a creatine kinase isoenzyme that plays a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine.

References

Haas RC. et al., 1989, J Biol Chem. 264 (5): 2890-7.
Stachowiak O. et al., 1998, Mol Cell Biochem. 184 (1-2): 141-51.
Lipskaya TY. 2001, Biochemistry Mosc. 66 (10): 1098-111.