MSN / Moesin (aa 1-346) Protein (His Tag)

07.06.2012

Produkt-Information von MSN / Moesin Protein

Synonym	MSN
Protein Construction	A DNA sequence encoding the human MSN (P26038) (Met 1-Glu 346) was expressed, with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von MSN / Moesin Protein

Purity	> 80 % as determined by SDS-PAGE	SDS-PAGE: MSN / Moesin protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human MSN consists of 361 amino acids and has a calculated molecular mass of 42.8 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris 0.5M NaCl pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MSN / Moesin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MSN / Moesin Protein

Moesin is a member of the ERM family which includes ezrin and radixin. It is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. ERM proteins, highly related members of the larger protein 4.1 superfamily, can exist in an active or inactive conformation. It seems that ERM proteins function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin contains 1 FERM domain and is expressed in all tissues and cultured cells studied. Moesin has been shown to interact with CD43, Neutrophil cytosolic factor 1, VCAM-1, Neutrophil cytosolic factor 4, ICAM3 and EZR. It probably involved in connections of major cytoskeletal structures to the plasma membrane. It also may inhibit herpes simplex virus 1 infection at an early stage. It also functions to promote cortical actin assembly and apical-basal polarity. As a result, cells lacking Moesin lose epithelial characteristics and adopt invasive migratory behaviour.

References

Lankes WT. et al., 1991, Proc Natl Acad Sci. 88 (19): 8297-301.
Serrador J M. et al., 1998, Blood. 91 (12): 4632-44.
Barreiro Olga. et al., 2002, J Cell Biol. 157 (7): 1233-45.

Sino Biological Inc. liefert MSN / Moesin (aa 1-346) Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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