CLPS / Colipase Protein (His Tag)

07.06.2012

Produkt-Information von CLPS / Colipase Protein

Synonym	CLPS
Protein Construction	A DNA sequence encoding the human CLPS (P04118) （Met 1-Gln 112）was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CLPS / Colipase Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: CLPS / Colipase protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 18
Molecular Mass	The recombinant human CLPS consists of 105 amino acids and predicts a molecular mass of 11.5 kDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, 500mM Nacl, pH 7.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CLPS / Colipase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CLPS / Colipase Protei

Colipase is a member of the colipase family. It is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.

References

Davis RC. et al., 1991, Genomics. 10 (1): 262-5.
Lowe ME. 1997, Annu Rev Nutr. 17: 141-58.
Verger R. et al., 1999, Biochim Biophys Acta. 1441 (2-3): 173-84.

Sino Biological Inc. liefert CLPS / Colipase Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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