Mouse TIMD3 / TIM3 / HAVCR2 Protein (His Tag)

16.04.2013

Sino Biological Inc. liefert Mouse TIMD3 / TIM3 / HAVCR2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse TIMD3 / TIM3 / HAVCR2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/TIM-3-HAVCR2-Protein-Antibody-a-694.html

Produkt-Information von TIMD3 / TIM3 / HAVCR2 Protein

Synonym	HAVCR2
Protein Construction	A DNA sequence encoding the mouse HAVCR2 (AAL65156.1) (Met1-Arg191) was expressed with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

TIMD3 / TIM3 / HAVCR2 Protein QC Testing

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: TIMD3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 22
Molecular Mass	The recombinant mouse HAVCR2 comprises 181 amino acids and has a predicted molecular mass of 20.3 kDa. The apparent molecular mass of the protein is approximately 37-47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von TIMD3 / TIM3 / HAVCR2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TIMD3 / TIM3 / HAVCR2 Protein

Hepatitis A virus cellular receptor 2 ( HAVCR2 ), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. TIM-3 thus serves as the surface molecule that distinguishes between the two effector populations derived from naive CD4(+) T-helper cells. TIM-3 is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. Th1 cells produce cytokines ( IFN-gamma, interleukin-2, TNF-alpha and lymphotoxin ) that are commonly associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity reactions, and induction of organ-specific autoimmune diseases. It has been identified that galectin-9 is the Tim-3 ligand, and the TIM-3-galectin-9 pathway may serve to inhibit Th1 cells during a normal immune response and may be crucial for the induction of peripheral tolerance. Dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

References

Monney L. et al., 2002, Nature. 415: 536-41.
Sabatos CA. et al., 2003, Nat Immunol. 4: 1102-10.
Zhu C. et al., 2005, Nat Immunol. 6: 1245-52.
Koguchi K. et al., 2006, J Exp Med. 203: 1413-8.
Anderson AC. et al., 2006, Curr Opin Immunol. 18: 665-9.