Mouse S100B / S100beta Protein

16.11.2012

Sino Biological Inc. liefert Mouse S100B / S100beta Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse S100B / S100beta Protein lesen Sie bitte: http://www.sinobiological.com/S100B-S100beta-Protein-g-9879.html

Produkt-Information von S100B / S100beta Protein

Synonym	S100B
Protein Construction	A DNA sequence encoding the mouse S100B (P50114)(Ser2-Glu92) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Source	Mouse
Expression Host	Human Cells

QC Testing von S100B / S100beta Protein

Purity	> 80 % as determined by SDS-PAGE	SDS-PAGE: S100B protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant mouse S100B/Fc comprises 351 amino acids and has a predicted molecular mass of 39 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von S100B / S100beta Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von S100B / S100beta Protein

S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. Although most of the S100 genes are located as a cluster at 1q21, S100B gene is located at 21q22.3. S100B is glial-specific and is synthesized in considerable amounts in the cytoplasm of astrocytes. This protein has been revealed to preform a variety of intracellular functions including cell proliferation, cell structure, energy metabolism, and calcium homeostasis. S100B is probably implicated in tumor progression by excessive down-regulation of the p53 tumor suppressor protein. As a neurotrophic factor, S100B stimulates neurite outgrowth and enhances survival of neurons during CNS development. Chromosomal rearrangements and abberant expression of S100B gene are associated with several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

References

Allore R. et al., 1988, Science. 239: 1311-1313.
Esposito G. et al., 2008, J Cell Mol Med. 12: 914-927.
Rothermundt M. et al., 2003, Microsc Res Tech. 60: 614-632.
Lin J. et al., 2004, J Biol Chem. 279: 34071-34077.
Nishiyama H. et al., 2002, Proc Natl Acad Sci. 99: 4037-4042.