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Mouse RPE / RPE2-1 Protein (His Tag)

Sino Biological Inc. liefert Mouse RPE / RPE2-1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse RPE / RPE2-1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/RPE-RPE2-1-Protein-g-9878.html

Produkt-Information von RPE / RPE2-1 Protein

Synonym RPE
Protein Construction

A DNA sequence encoding the mouse RPE (Q8VEE0) (Met1-Arg228) was expressed with a C-terminal polyhistidine tag.

Source Mouse
Expression Host Human Cells

QC Testing von RPE / RPE2-1 Protein

Purity >90% as determined by SDS-PAGE SDS-PAGE:
SDS-PAGE

RPE protein

Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Stability Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal Met
Molecular Mass

The recombinant mouse RPE comprises 239 amino acids and has a predicted molecular mass of 26.4 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RPE / RPE2-1 Protein

Storage Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von RPE / RPE2-1 Protein

Related Areas:

Enzyme>>Carbohydrate Metabolism Enzymes>>RPE

Proteins:
Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
RPE Human RPE Protein, Recombinant 11970-H07H
RPE Human RPE Protein, Recombinant 11970-H08H
RPE Mouse RPE / RPE2-1 Protein, Recombinant 50968-M08H
Antibodies:

Beschreibung von RPE / RPE2-1 Protein

The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylase (OMPDC), and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. In enzymology, a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence, RPE has one substrate, L-ribulose 5-phosphate, and one product, L-xylulose 5-phosphate. RPE belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase, UlaE, and SgaU.

References

  1. Yew WS. et al., 2002, J Bacteriol. 184 (1): 302-6.
  2. Shimaoka M. et al., 2005, Biosci Biotechnol Biochem. 69 (7): 1248-55.
  3. Salinero KK. et al., 2009, BMC Genomics. 10: 351.
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