Mouse S100A8 / CAGA Protein (His Tag)

21.05.2013

Sino Biological Inc. liefert Mouse S100A8 / CAGA Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse S100A8 / CAGA Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/S100A8-Protein-Antibody-a-979.html

Produkt-Information von S100A8 / CAGA Protein

Synonym :	S100a8, Caga, Mrp8
Protein Construction:	A DNA sequence encoding the mouse S100a8 (P27005) (Pro2-Glu89) was expressed, with a polyhistidine tag at the N-terminus.
Source:	Mouse
Expression Host:	E.coli

QC Testing von S100A8 / CAGA Protein

Purity:	> 95 % as determined by SDS-PAGE	SDS-PAGE: S100A8 protein
Endotoxin:	Please contact us for more information.
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	His
Molecular Mass:	The recombinant mouse S100a8 consists of 104 amino acids and predicts a molecular mass of 12.3 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
Formulation:	Lyophilized from sterile 50mM Tris, 100mM NaCl, 20% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von S100A8 / CAGA Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von S100A8 / CAGA Protein

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A8, also known as S100 calcium-binding protein A8, Cystic fibrosis antigen, Migration inhibitory factor-related protein 8, S100A8, and CAGA, is a member of the S-100 family. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 plays an important role in dedifferentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer.

References

Donato R. et al., 2003, Microsc Res Tech. 60 (6): 540-51.
Gebhardt C. et al., 2006, Biochem Pharmacol. 72 (11): 1622-31.
Nonaka D. et al., 2008, J Cutan Pathol. 35 (11): 1014-9.
Lim SY. et al., 2008, J Immunol. 181 (8): 5627-36.