JAM3 / JAM-C Protein

21.05.2013

Sino Biological Inc. liefert JAM3 / JAM-C Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über JAM3 / JAM-C Protein lesen Sie bitte: http://www.sinobiological.com/JAM-C-JAM3-Protein-a-1281.html

Produkt-Information von JAM3 / JAM-C Protein

Synonym	JAM3
Protein Construction	A DNA sequence encoding the human JAM3 (Q9BX67) (Met 1- Asn 241) was expressed with six amino acids (LEVLFQ) at the C- terminus was expressed and purified.
Source	Human
Expression Host	Human Cells

QC Testing von JAM3 / JAM-C Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: JAM3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 32
Molecular Mass	The recombinant human JAM3 consists of 217 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 33 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von JAM3 / JAM-C Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von JAM3 / JAM-C Protein

Junctional adhesion molecule C, also known as Junctional adhesion molecule 3, JAM3, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. JAM3 is widely expressed. The highest expression of JAM3 is in placenta, brain and kidney. JAM3 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. At the protein level JAM3 displays 36 and 32% identity to JAM2 and JAM1, respectively. JAM3 participates in cell-cell adhesion distinct from tight junctions. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. Unlike other proteins in the same family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions.

References

Suzuki Y. et al.,1997, Gene. 200 (1-2): 149-56.
Arrate MP. et al., 2001, J Biol Chem. 276 (49): 45826-32.
Cunningham SA. et al., 2002, J Biol Chem. 277 (31): 27589-92.
Muller WA. et al., 2003, Trends Immunol. 24 (6): 327-34.