Mouse CDC2 Kinase / CDK1 Protein (GST Tag)

22.05.2013

Sino Biological Inc. liefert Mouse CDC2 Kinase / CDK1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse CDC2 Kinase / CDK1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/CDC2-CDK1-Protein-a-1167.html

Produkt-Information von CDK1 / CDC2 Protein

Synonym	CDK1, Cdc2, Cdc2a, Cdkn1
Protein Construction	A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von CDK1 / CDC2 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: CDK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse CDK1/GST chimera consists of 534 amino acids and has a calculated molecular mass of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CDK1 / CDC2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CDK1 / CDC2 Protein

Cell division control protein 2 homolog, also known as p34 protein kinase, Cyclin-dependent kinase 1, CDK1 and CDC2, is a nucleus protein which belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family. CDC2 / CDKX subfamily. CDK1 / CDC2 contains one protein kinase domain. CDK1 / CDC2 forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. CDK1 / CDC2 interacts with DLGAP5. Isoform 2 of CDK1 / CDC2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. CDK1 / CDC2 interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. CDK1 / CDC2 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Expression level of CDK1 increased along with the malignant progression of ganglioglioma, and decreased with the differentiation process of neural stem cells. Expression of CDK1 was high in human fetal brain tissues and bone marrows of nude mice, but low in normal adult human brain tissues. Overexpression of CDK1 may promote oncogenesis and progression of human gliomas. Downregulation of CDK1 expression can inhibit the proliferation activities of human malignant gliomas.

References

Ubersax JA. 2003, Nature. 425 (6960): 859-64.
Frank CJ. 2006, Mol Cell. 24 (3): 423-32.
Chen H. 2007, Zhonghua Zhong Liu Za Zhi. 29 (7): 484-8.
Moseley JB. 2009, Curr Opin Cell Biol. 21 (1): 82-8.