Mouse R-Spondin 1 / RSPO1 Protein (His Tag)

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Produkt-Information von R-Spondin 1 / RSPO1 Protein

QC Testing von R-Spondin 1 / RSPO1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: RSPO1 protein
Bio-activity	Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 21
Molecular Mass	The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von R-Spondin 1 / RSPO1 Protein

Verwandte Produkte & Themen von R-Spondin 1 / RSPO1 Protein

Beschreibung von R-Spondin 1 / RSPO1 Protein

R-Spondin 1 ( Roof plate-specific Spondin 1 ), also known as RSPO1 and Cristin 3, is a secreted protein which belongs to the R-Spondin family. The R-Spondin (RSPO) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. R-Spondin1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mature human R-Spondin1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. Human R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin1, respectively. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY.

References

1. Parma, P. et al., 2006, Nature genetics. 38 (11): 1304-9.
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3. Binnerts, ME. et al., 2007, Proc Natl Acad Sci. 104 (37): 14700-5.
4. Wilhelm, D., 2007, Bioessays. 29 (4): 314-8.
5. Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6.