Mouse PTPN6 (aa 207-597) Protein (GST Tag)

08.03.2013

Sino Biological Inc. liefert Mouse PTPN6 (aa 207-597) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse PTPN6 (aa 207-597) Protein (GST Tag) lesen Sie bitte:http://www.sinobiological.com/PTPN6-Protein-g-10557.html

Produkt-Information von PTPN6 Protein

Synonym	PTPN6, Hcp, Hcph, Ptp1C
Protein Construction	A DNA sequence encoding the mouse PTPN6 (P29351-2) (Ala207-Lys597) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von PTPN6 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: PTPN6 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse PTPN6/GST chimera consists of 628 amino acids and has a calculated molecular mass of 72.7 kDa. The recombinant protein migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PTPN6 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTPN6 Protein

PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

References

Yu CL. et al., 2000, J Biol Chem. 275 (1): 599-604.
Wu DW. et al., 2000, Blood Cells Mol Dis. 26 (1): 15-24.
Jiao H. et al., 1996, Mol Cell Biol. 16 (12): 6985-92.