Mouse Frizzled-5 / FZD5 Protein

07.03.2013

Sino Biological Inc. liefert Mouse Frizzled-5 / FZD5 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse Frizzled-5 / FZD5 Protein lesen Sie bitte: http://www.sinobiological.com/Frizzled-5-FZD5-Protein-g-10554.html

Produkt-Information von Frizzled-5 / FZD5 Protein

Synonym	FZD5
Protein Construction	A DNA sequence encoding the mouse FZD5 (Q9EQD0) (Met1-Pro167) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Mouse
Expression Host	CHO Stable Cell

QC Testing von Frizzled-5 / FZD5 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: Frizzled-5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 27
Molecular Mass	The recombinant mouse FZD5 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 382 amino acids and has a predicted molecular mass of 42.9 kDa. The apparent molecular mass of the protein is approximately 52-56 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Frizzled-5 / FZD5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Frizzled-5 / FZD5 Protein

Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 10 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. Frizzled-5 / FZD5 is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt10B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, Frizzled-5 / FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.

References

Cadigan KM. et al., 1997, Genes Dev. 11: 3286-305.
Liu C. et al., 2008, J. Neurosci. 28: 5641-53.
Caricasole A. et al., 2003, J. Biol. Chem. 278:37024-31.
Blumenthal A. et al., 2006, Blood.108: 965-73.