Mouse MAPK1 / ERK2 Protein

26.04.2013

Sino Biological Inc. liefert Mouse MAPK1 / ERK2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse MAPK1 / ERK2 Protein lesen Sie bitte: http://www.sinobiological.com/ERK2-MAPK1-MAPK2-Protein-a-853.html

Produkt-Information von MAPK1 / ERK2 Protein

Synonym	ERK; Erk2; MAPK2; PRKM2; Prkm1
Protein Construction	A DNA sequence encoding the mouse MAPK1(P63085) (Met1-Ser358) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von MAPK1 / ERK2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: MAPK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The recombinant mouse MAPK1 consists of 360 amino acids and predicts a molecular mass of 41.4 KDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von MAPK1 / ERK2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MAPK1 / ERK2 Protein

Mitogen-activated protein kinase 1, also known as Extracellular signal-regulated kinase 2, ERK-2, and MAPK1, is a member of the protein kinase superfamily and MAP kinase subfamily. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity.

References

Formstecher E. et al., 2001,Dev Cell. 1 (2): 239-50.
Xu Be. et al., 2001, J Biol Chem. 276 (28): 26509-15.
Slack DN. et al., 2001, J Biol Chem. 276 (19): 16491-500.
Calvisi DF. et al., 2008, Cancer Res. 68 (11): 4192-200.