Mouse MAP2K1 / MEK1 / MAPKK1 Protein

26.04.2013

Sino Biological Inc. liefert Mouse MAP2K1 / MEK1 / MAPKK1 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse MAP2K1 / MEK1 / MAPKK1 Protein lesen Sie bitte: http://www.sinobiological.com/MEK1-MAP2K1-MKK1-Protein-a-2439.html

Produkt-Information von MAP2K1 / MEK1 / MAPKK1 Protein

Synonym	MAP2K1, Mek1, Prkmk1
Protein Construction	A DNA sequence encoding the mouse MAP2K1 (NP_032953.1) (Met1-Ile393) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von MAP2K1 / MEK1 / MAPKK1 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: MAP2K1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The recombinant mouse MAP2K1 consists of 395 amino acids and predicts a molecular mass of 43.6 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von MAP2K1 / MEK1 / MAPKK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MAP2K1 / MEK1 / MAPKK1 Protein

Dual specificity mitogen-activated protein kinase kinase 1, also known as MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAP2K1 and MEK, is a protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MEK1/MAP2K1/MKK1 is widely expressed, with extremely low levels in brain. MEK1/MAP2K1/MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1/MAP2K1/MKK1 activates ERK1 and ERK2 MAP kinases. Defects in MEK1/MAP2K1/MKK1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) which also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy.

References

Seger R. et al., 1992, J Biol Chem. 267: 25628-31.
Duesbery NS. et al., 1998, Science. 280: 734-7.
Mukherjee S. et al., 2006, Science. 312: 1211-4.
Wissing J. et al., 2007, Mol Cell Proteomics. 6: 537-47.