Mouse CDC37 / CDC37A Protein (GST Tag)

20.05.2013

Sino Biological Inc. liefert Mouse CDC37 / CDC37A Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse CDC37 / CDC37A Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/CDC37-Protein-a-1329.html

Produkt-Information von CDC37 / CDC37A Protein

Synonym	CDC37
Protein Construction	A DNA sequence encoding the mouse CDC37 (Q61081) (Met1-Ala379) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von CDC37 / CDC37A Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: CDC37 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse CDC37/GST chimera consists of 616 amino acids and has a calculated molecular mass of 72.4 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CDC37 / CDC37A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CDC37 / CDC37A Protei

Hsp90 co-chaperone CDC37, also known as Hsp90 chaperone protein kinase-targeting subunit, cell division cycle 37 homolog, CDC37 and CDC37A, is a cytoplasm protein which belongs to the CDC37 family. CDC37 is monomeric in its physiological state, and that it becomes a dimer only when it is complexed with both Hsp90 and protein kinases. CDC37 is a co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. CDC37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. CDC37 is not an inhibitor nor an activator of the Cdk4 / cyclin-D1 kinase, while it appears to facilitate complex assembly between Cdk4, and cyclin-D1. CDC37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via CDC37. CDC37 is a cell division cycle control protein of Saccharomyces cerevisiae. It is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK, as well as eIF-2 alpha kinases. CDC37 is thought to play a critical role in directing Hsp90 to its target kinases.

References

Lamphere L. et al., 1997, Oncogene. 14 (16): 1999-2004.
Pearl LH. et al., 2005, Curr Opin Genet Dev. 15 (1): 55-61.
Miyata Y. et al., 2005, Mol Cell Biochem. 274 (1-2): 171-9.
Roiniotis J. et al., 2005, Biochemistry. 44 (17): 6662-9.
Terasawa K. et al., 2006, Biosci Biotechnol Biochem. 70 (6): 1542-6.