Mouse IL18 / IL-18 Protein

01.03.2013

Produkt-Information von IL18 / IL-18 Protein

Synonym :	IL18, Igif
Protein Construction:	A DNA sequence encoding the mouse IL18 (P70380) (Asn36-Ser192) was expressed and purified.
Source:	Mouse
Expression Host:	E.coli

QC Testing von IL18 / IL-18 Protein

Purity:	> 90 % as determined by SDS-PAGE	SDS-PAGE: IL18 / IL-18 protein
Endotoxin:	Please contact us for more information.
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Met
Molecular Mass:	The recombinant mouse IL18 consists of 158 amino acids and predicts a molecular mass of 18.2 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
Formulation:	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IL18 / IL-18 Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IL18 / IL-18 Protein

Interleukin-18, also known as Interferon gamma-inducing factor, Iboctadekin, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma IL1F4 and IL18, is a secreted protein which belongs to the IL-1 family. IL-18 / Interleukin-18 is related to the IL1 family by structure, receptors, signalling molecules, and function. IL18 induces gene expression and synthesis of tumour necrosis factor (TNF), IL1, Fas ligand, several chemokines, and vascular adhesion molecules. Similar to IL-1beta, IL-18 / Interleukin-18 is synthesised as a biologically inactive precursor molecule lacking a signal peptide. IL-18 / Interleukin-18 augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Rheumatoid arthritis (RA) is a complex autoimmune disease with a strong genetic contribution to its pathogenesis. Interleukin-18 (IL-18) is a proinflammatory cytokine involved in the pathogenesis of RA. IL-18 / Interleukin-18 is also a proinflammatory cytokine whose levels are increased in the subclinical stage of insulin-dependent (type I) diabetes mellitus.

References

Dinarello CA. 2001, Ann Rheum Dis. 60 Suppl 3: iii18-24.
Liew FY. et al., 2002, Ann Rheum Dis. 61 Suppl 2: ii100-2.
Martin RJ. et al., 2005, Genes Immun. 6 (2): 171-4.
Sakai K. et al., 2008, J Infect Dis. 197 (12): 1752-61.
Pawlik A. et al., 2009, Scand J Rheumatol. 38 (3): 159-65.

Cytokine