Mouse IL-23 / IL23A & IL12B Heterodimer Protein (His Tag)

16.05.2013

Sino Biological Inc. liefert Mouse IL-23 / IL23A & IL12B Heterodimer Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse IL-23 / IL23A & IL12B Heterodimer Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/IL-23-IL23A-IL12B-Heterodimer-Protein-g-11715.html

Produkt-Information von IL-23 / IL23A & IL12B Heterodimer Protein

Synonym	Interleukin-23 (IL23A & IL12B Heterodimer)
Protein Construction	A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.
Source	Mouse
Expression Host	Human Cells

QC Testing von IL-23 / IL23A & IL12B Heterodimer Protein

Purity	(33.1+31.5+32.9) % as determined by SDS-PAGE	SDS-PAGE IL23A & IL12B Heterodimer protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 22 & Met 23
Molecular Mass	The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von IL-23 / IL23A & IL12B Heterodimer Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IL-23 / IL23A & IL12B Heterodimer Protein

Interleukin-23 subunit alpha, also known as IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19 and IL23A, is a secreted protein which belongs to the IL-6 superfamily. IL-23A associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Interleukin-12 subunit beta, also known as cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2, NKSF2 and IL12B, is secreted protein which belongs to the type I cytokine receptor family and Type 3 subfamily. IL12B / NKSF2 is a ctokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. IL12B / NKSF2 associates with IL-23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues.

References

Altare F. et al., 1998, J Clin Invest. 102: 2035-40.
Oppmann B. et al., 2000, Immunity. 13: 715-25.
Picard C. et al., 2002, Am J Hum Genet. 70: 336-48.
Parham C. et al., 2002, J Immunol. 168: 5699-708.
Pirhonen J. et al., 2002, J Immunol. 169: 5673-8.