Mouse GM-CSF / CSF2 Protein

09.06.2013

Sino Biological Inc. liefert Mouse GM-CSF / CSF2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse GM-CSF / CSF2 Protein lesen Sie bitte: http://www.sinobiological.com/GM-CSF-Protein-a-417.html

Produkt-Information von GM-CSF / CSF2 Protein

Synonym	CSF2, Csfgm
Protein Construction	A DNA sequence encoding the mouse CSF2 (P01587) (Ala18-Lys141) was was expressed with the fused Fc region of human IgG1 at the N-terminus.
Source	Mouse
Expression Host	Human Cells

QC Testing von GM-CSF / CSF2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE GM-CSF protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant mouse CSF2/Fc comprises 384 amino acids and has a predicted molecular mass of 42.5 kDa. The apparent molecular mass of the protein is approximately 44-52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GM-CSF / CSF2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GM-CSF / CSF2 Protein

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells. GM-CSF is secreted by a variety of cell types including macrophages, T cells, mast cells, endothelial cells and fibroblasts. This cytokine has a central role in governing the proliferation, maturation, differentiation and function of granulocyte and macrophage lineage populations, as well as the migration of endothelial cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target.

References

Frolova EI. et al., 1991, Proc Natl Acad Sci. 88: 4821-4.
Hamilton JA. et al., 2002, Trends Immunol. 23: 403-8.
Ebner K. et al., 2003, Blood. 102: 192-9.
Kato T. et al., 2008, J Interferon Cytokine Res. 28: 235-43.
Volmar CH. et al., 2008, Cytokine. 42: 336-44.